Figure 6
Effect of Hex, Chl and MeOH extracts on caspase-3 activity in K562 cells. Lysates prepared from cells treated with N. retusa leaf extracts for 24 h and 48 h, were assayed for in vitro caspase-3 activity. The rate of cleavage of the caspase substrate DEVD-p NA was measured at 405 nm. The results are presented as the mean ± SD. The experiments were done in triplicate. (*) p < 0.05 means a significant difference between the control and treated cells Control: cells treated by the vehicle only.