Stimulation of sEPCR release in normal PrEC and prostate cancer cell lines by pharmacological agents and pro-inflammatory cytokines. Cells were incubated for 1 h either with 100 ng/ml PMA, 3 μM ionomycin, 0.8 mM H2O2, and 4 mM MβCD (A) or with pro-inflammatory cytokines (IFN-γ, IL-1β, IL-6, and TNF-α) at final concentrations of 25 ng/ml (B). Released sEPCR in cell culture medium was determined using sEPCR ELISA technique. Results are the means ± SDs of analyses in triplicates and are representative of two similar experiments. * p < 0.05 versus controls without cytokines.