Chemotherapy induces the tyrosine kinase cascade mRNA expression in HCC cells. Gemcitabine (11.4 μg/ml or 114 μg/ml) or Doxorubicin (0.15 μg/ml or 1.5 μg/ml) was added to HepG2 cells twice weekly and cultured in RPMI 1640 containing 10% FBS. A: Tyrosine kinase mRNA expression was assessed by RT-PCR. Columns average of three independent experiments, bars SD. B: protein expression of tyrosine kinases was assessed by Western blot. *p ≤ 0.05; **p ≤ 0.001 compared to the untreated control group.