Figure 3

EGF stimulated activation of the tyrosine kinase pathway increases the ABC-transport protein mRNA expression and cellular proliferation of resistant HepG2 cells. Resistant HCC cells were cultured in RPMI 1640 and treated with EGF 500 ng/ml for 24 hours. A: Tyrosine kinase mRNA expression was assessed by RT-PCR. Columns average of three independent experiments, bars SD. B: ABC-transport protein mRNA expression was assessed by RT PCR. Columns average of three independent experiments, bars SD. C: Cellular viability was analyzed by MTT assay. A minimum of three independent experiments was performed. *p ≤ 0.05 compared to the untreated control group.