Real-time RT-PCR analysis of ENO-2 mRNA levels in parental MCF-10A cells treated with epigenetic regulators. Figure 10 (A) shows expression of ENO-2 mRNA after treatment with histone deacetylase Inhibitor, MS-275 for up to 72 hrs and (B) shows expression of ENO-2 mRNA after treatment with the DNA demethylation agent, 5-AZC, for 24 hrs. Statistical analysis consisted of ANOVA with Tukey post hoc testing performed using GraphPad PRISM 5. *Denotes a statistically significant difference from untreated MCF-10A parent cells (p < 0.05). Real-time data is plotted as the mean ± SEM of triplicate determinations.