Figure 3

Expression of ENO-2 mRNA and protein in parental MCF-10A cells exposed to As⁺³ and Cd⁺². Cell viability, as an indicator of cytotoxicity, was determined by measuring the capacity of the MCF-10 cells to reduce MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) to formazan after exposure to As⁺³ (A) and Cd⁺² (B). Real-time RT-PCR analysis of ENO-2 mRNA in parental MCF-10A cells exposed to As⁺³ and Cd⁺² (C and F). Western blot analysis of ENO-2 protein in parental MCF-10A cells exposed to As⁺³ and Cd⁺² (D and G) and western blot densitometric analysis (E and H) respectively. Statistical analysis consisted of ANOVA with Tukey post hoc testing. *Denotes a statistically significant difference from untreated MCF-10A parent cells (p < 0.05). Real-time data is plotted as the mean ± SEM of triplicate determinations.