Figure 3

Cell cycle analysis after incubation with Eag and HERG blockers. Effect of Eag blockers (imipramine and clofilium) and HERG blockers (E-4031 and ergtoxin) on SK-OV-3 cells. E-4031 (20 μM) shows a block after S phase leading to increased cells in the S phase (Figure 3B) while ergtoxin (100ηM) has an effect on G2/M cell cycle checkpoint leading to the accumulation of cells in G2/M and S phase and less cells going through to G1 phase (Figure 3A-C) (* P < 0.05, ** P < 0.01). Imipramine (50 μM) and clofilium (3300ηM) showed no effect on cell cycle.