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Figure 5 | Cancer Cell International

Figure 5

From: 5-alpha-reductase type I (SRD5A1) is up-regulated in non-small cell lung cancer but does not impact proliferation, cell cycle distribution or apoptosis

Figure 5

Immunoblot after over-expression of SRD5A1-V5 in A549 cells and subsequent siRNA transfection. Lysates were prepared 24 h after siRNA transfection, The percentage signal intensity compared to lane 7 is given in brackets. The 25 kDa band corresponds to SRD5A1-V5, the 37 kDa band to GAPDH. Lane 1: siRNA 1 (31%); lane 2: mm 1 (104%); lane 3: siRNA 2 (22%); lane 4: mm 2 (87%); lane 5: siRNA 3 (29%); lane 6: mm 3 (107%); lane 7: plasmid (w/o subsequent siRNA treatment) (100%); lane 8: untreated (0%).

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