DNA and mRNA analysis. A) RT-PCR performed on total RNA extracted from cells at d1 (24 hr) following FA administration [400 μg/mL] revealed little change in p53 mRNA transcription in HGF-1 cells, but decreased transcription in CAL27, SCC15, and SCC25 cells. HPV16 and HPV18 had little effect on FA-induced changes in p53 transcription in CAL27 and SCC25 cells, although decreases were observed in both SCC15 and HGF-1 cells. B) PCR screening from DNA confirmed HPV16 and HPV18 DNA was present in the control cell lines, CaSKi (HPV16) and GH354 (HPV18) cervical adenocarcinomas, but not HGF-1, SCC25, SCC15 or CAL27. RT-PCR screening also confirmed the presence of HPV-specific mRNA in the control, but not experimental, cell lines. C) DNA screening post-transfection confirmed the presence of HPV-specific DNA in CAL27, SCC25, SCC15 and HGF-1, but not mock transfectants (mTF); RNA screening confirmed production of HPV-specific mRNA at levels similar to control cell lines.