Figure 9

Molecular network of downregulated and upregulated genes in U373MG cells following exposure to the compounds 1a and 3. Entrez Gene IDs of the combined set of 317 downregulated genes and 30 upregulated genes in U373MG cells shared by the compounds 1a and 3 were imported into KeyMolnet. The neighboring network-search algorithm on core contents illustrated the highly complex molecular network showing the most significant relationship with transcriptional regulation by Rb/E2F. Red or blue nodes represent upregulated or downregulated genes, respectively. White nodes exhibit the molecules extracted automatically from the core contents of KeyMolnet to establish molecular connections. The molecular relation is indicated by solid line with arrow (direct binding or activation), solid line with arrow and stop (direct inactivation), solid line without arrow (complex formation), dash line with arrow (transcriptional activation), and dash line with arrow and stop (transcriptional repression). The red circle indicates the E2F family of transcription factors.