Anticonvulsant and analgesic activities of crude extract and its fractions of the defensive secretion from the Mediterranean sponge, Spongia officinalis

Table 1 Anticonvulsant effect of the subcutaneous administration of crude extract and its semi-purified fractions (F1-F3) of the defensive secretion of Spongia officinalis in the PTZ model in mice

Treatment	Dose (mg/kg)	Onset of first clonus (s)	Duration (s)	Seizure protection (%)	Mortality protection (%)
Control (saline 10 ml/kg)	-	180.16 ± 53.66	15 ± 1.16	0	0
Crude extract	100	149 ± 35.77^ns	12 ± 87.24^ns	0	0
	200	251 ± 17.88 ^ns	10.7 ± 3.57^ns	0	0
	400	600 ± 0^**	5 ± 2.68^**	30	0
F1	200	251 ± 9.83^*	18 ± 3.57^ns	0	o
F2	50	247 ± 17.88 ^ns	8.7 ± 1.97^**	0	0
	100	800 ± 17.88^**	4 ± 0.71^**	20	0
	200	1200 ± 89.44^**	1 ± 0.89^**	50	30
F3	200	249 ± 10.73 ^ns	13 ± 1.07^ns	0	0
Phenobarbital (reference drug)	120	-	-	100	100

Values are expressed as mean ± s.e.m. *P < 0.01, **P < 0.001, ns: not significant. n = 6 animals.

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