Scoring system used to evaluate paralemmin-1 expression in TMA from breast tumors and reduction mammoplasty specimens. Intensity of immunoreactivity of epithelial cells was used to assign scores of 0, 1, 2, and 3. A 0 was little to no staining, a 1 was considered to be “light” intensity of staining; a 2 was considered to be “moderate” intensity of staining, a 3 was considered to be “strong” intensity of staining. Images are from a DCIS TMA with inset in score 1, 2 and 3 images showing increasing intensity of paralemmin-1 plasma membrane staining in tumor cells. Image for score 0 shows negative paralemmin-1 staining in tumor cells and small peritumoral lymphocytes (left). Intratumoral and peritumoral vascular endothelial cells (*) and elongated stromal cells are positive for paralemmin-1. Scale bars 50 micron.