Table 3 Primers used to detect exon-deleted splice variants in paralemmin-1
From: Paralemmin-1 is over-expressed in estrogen-receptor positive breast cancers
Primer Sequence | Nucleotide start site | Product Lengths (bp) | |||
|---|---|---|---|---|---|
Full | Deleted | ||||
PS4 | sense | AGGCGGAGATCGAGAACAAG | 282 | 221 | 90 |
antisense | CCAGCACCTCAATTTCCTTC | 503 | |||
PS5 | sense | CAGGACGACGAGCAGAAGA | 431 | 233 | 82 |
antisense | GGAGACTCGCTTGTCTTTGG | 664 | |||
PS6 | sense | AAGGAAATTGAGGTGCTGGA | 485 | 185 | 163 |
antisense | CGTGTTGGAGACTCGCTTG | 670 | |||
PS7 | sense | TGATGAATTCACAGCAGACG | 612 | 150 | 90 |
antisense | GTCTCCCCTGTCACCTTGTC | 762 | |||
PS8 | sense | ACAAGCGAGTCTCCAACACG | 651 | 275 | 143 |
antisense | CCGCTTTGTGGATGAGTTC | 926 | |||