Figure 1From: Extracellular cyclophilin-A stimulates ERK1/2 phosphorylation in a cell-dependent manner but broadly stimulates nuclear factor kappa BExtracellular PPIA activity monitored through luciferase reporters assays. Sixteen luciferase reporters were used to monitor the response to extracellular PPIA in several cell lines, which included the following: A) HEK293T cells, B) MOLM13 cells, C) PANC-1 cells, and D) L3.6PL. Cells were transiently transfected with the indicated reporters and then stimulated with recombinant 25 μM PPIA as described in Materials and Methods. Luciferase activity is shown as a fold-increase over the buffer control (Luc Fold-Increase). Dashed line (grey) indicate no Luc Fold-Increase (i.e., unity). *Beta-Catenin (βCate) and *HRE refer to plasmids that comprise the transcription target sites for HIF and βCate transcription factors, respectively. The remaining plasmids comprise the indicated promoters for each protein. Bars indicate the standard error of two replicates.Back to article page