Figure 3

Characterizing extracellular PPIA-mediated activity of NFκB within multiple cell lines. A) An NFκB translocation assay was conducted in several adherent cell lines, which included HEK293T cells (top), PANC-1 cells (middle), and L3.6pL cells (bottom) with the addition of either buffer control or PPIA. Cells were transiently transfected with the p65-GFP plasmid (green, left panels) as well as DAPI stained (blue, middle panels) and the merge is shown (green, right panels). B) PPIA-induced luciferase reporter activity was monitored for the wild type IL-6 reporter (WT) and two mutations to the NFκB binding element (Mut1 and Mut2) for HEK293T and L3.6pL cells. C) PPIA-induced luciferase reporter activity was monitored for the wild type IL-8 luciferase reporter (WT) and two mutations within the NFκB binding element (also called Mut1 and Mut2) for MOLM13, PANC-1, and L3.6pL cells. PPIA-induced luciferase reporter activity was monitored as in Figure 1.