Figure 2

siRNA-Kaiso efficiently down-regulates cytoplasmic Kaiso expression in K562 cell line. (A). Immunofluorescence staining of Kaiso and β-tubulin. siRNA-Kaiso only efficiently downregulated cytoplasmic Kaiso expression in k562 cell line when compared to the scrambled knock-down cells (B). Cytoplasmic vs. nuclear fractionation Western blot analysis confirmed the results of immunofluorescence staining. β-tubulin served as an internal control. (C). Expression analysis of Kaiso knock-down by Real Time RT-PCR. (D). Expression analysis of p120ctn knock-down by Real Time RT-PCR. Data were expressed as mean ± standard deviation (S.D.). Columns, mean (n = 3); error bars, S.D.