Table 2 Primer design: oligo nucleotide sequences

Beta-Actin F

GCACCACACCTTCTACAATGAGC

Beta-Actin R

TTGAAGGTAGTTTCGTGGATGCC

Beta-tubulin III F

AACGAGGCGCTCTACGACATC

Beta-tubulin III R

CTCCTCCTCGTCGTCTTCGTA

Bmi-1AS

CATTGCTGCTGGGCATCGTAAG

Bmi-1 S

GGAGACCAGCAAGTATTGTCCTTTTG

BMP4 F

GTGAGGAGCTTCCACCACGA

BMP4 R

ACTGGTCCCTGGGATGTTCTC

Cathepsin B F

GCAGCCTCAGCCACCCAGAT

Cathepsin B R

CCACCATTACAGCCGTCCCCACAC

Cathepsin L F

CCGGGGAGGGCAGTTGAG

Cathepsin L R

CCTTGAGGCCCAGAGCAGTC

Cav-1 F

GGACATCTCTACACCGTTCCC

Cav-1 R

TTATATTTCTTTCTGCAAGTT

Cav-2 F

ATGGGGGCTGGAGACGGAGAAG

Cav-2 R

TCAATCCTGGCTCAGTTGCAG

CD133 AS

ACGCCTTGTCCTTGGTAGTGTTG

CD133 S

CTGGGGCTGCTGTTTATTATTCTG

c-Myc F

AAGGTCAGAGTCTGGATCAC

c-Myc R

TAACTACCTTGGGGGCCTTT

CXCR4 F

CACCGCATCTGGAGAACCA

CXCR4 R

GCCCATTTCCTCGGTGTAGTT

Dach1 S

AGGCTTTCGACCTGTTCCTGAA

Dach1 AS

GCTGTCAGACCTGTTGGTGGAA

DCX AS

GTTTCCCTTCATGACTCGGCA

DCX S

AATCCCAACTGGTCTGTCAAC

EPHB1 S

GAGATGGACAGCTCCAGAGG

EPHB1 AS

CCAGCATGAGCTGGTGTAGA

EPHB2 S

AAAATTGAGCAGGTGATCGG

EPHB2 AS

TCACAGGTGTGCTCTTGGTC

EPHB3 S

AGCAACCTGGTCTGCAAAGT

EPHB3 AS

TCCATAGCTCATGACCTCCC

EFNB1 S

GGAGGCAGACAACACTGTCA

EFNB1 AS

GAACAATGCCACCTTGGAGT

EFNB2 S

GCAAGTT-CTGCTGGATCAAC

EFNB2 AS

AGGATG-TTGTTCCCCGAATG

EFNB3, S

CTGAAATGCCCATGGAAAGA

EFNB3, AS

ACGCCCAGCAAGAGCAGCGC

GAPDH S

ACCACAGTCCATGCCATCAC

GAPDH AS

TCCA CCACCCTGTTGCTGTA

GFAP S

ACATCGAGATCGCCACCTAC

GFAP AS

ACATCACATCCTTGTGCTCC

HIF1 S

GTCGGACAGCCTCACCAAACAGAGC

HIF1 AS

GTTAACTTGATCCAAAGCTCTGAG

IGF1R S

ACGCCAATAAGTTCGTCCAC

IGF1R AS

TCCATCCTTGAGGGACTCAG

Ki67 F

GGAGGCAATATTACATAATTTCA

Ki67 R

CAGGGTCAGAAGAGAAGCTA

MMP2 F

CCA CGT GAC AAG CCC ATG GGG CCC C

MMP2 R

GCA GCC TAG CCA GTC GGA TTT GAT G

MMP9 F

CAACATCACCTATTGGATCC

MMP9 R

CGGGTGTAGAGTCTCTCGCT

MMP13 F

TGCTCGCATTCTCCTTCAGGA

MMP13 R

ATGCATCCAGGGGTCCTGGC

MMP14 F

CGCTACGCCATCCAGGGTCTCAAA

MMP14 R

CGGTCATCATCGGGCAGCACAAAA

msi1 S

GAGACTGACGCGCCCCAGCC

msi1 AS

CGCCTGGTCCATGAAAGTGACG

Mucin9 F

CATGACCGGTGGACTTTTCT

Mucin9 R

TCCTGTGAACCTTTCCCAAC

Mucin18 F

GTGTTGAATCTGTCTTGTGAA

Mucin18 R

ATGCCTCAGATCGATG

Mucin24 F2

GTTAATACTACCTGCTTTTGGATAGAATGT

Mucin24 R2

CCACTTGACAATCACTAACTGTTGAG

NCAM1 AS

GGTGTTGGAAATGCTCTGGT

NCAM1 S

AGGAGACAGAAACGAAGCCA

Nestin

GGCAGCGTTGGAACAGAGGTTGGA

Nestin R

CTCTAAACTGGAGTGGTCAGGGCT

Notch1 F

TGTTAATGAGTGCATCTCCAA

Notch1 R

CATTCGTAGCCATCAATCTTGTCC

NSE AS

GACAGTTG CAGGCCTTTTCTTC

NSE S

CATCGA CAAGGCTGGCTACACG

Nucleostamin F

CATGACCTGCCATAAGCGGT

Nucleostamin R

CAATTACTCCAACCCGAATGGC

Pax6 F

GAGCCTCATCTGAATCTTCTCCG

Pax6 R

CGTCCATCTTTGCTTGGGAAATC

p53 S

TTGGATCCATGTTTTGCCAACTGGCC

p53 AS

TTGAATTCAGGCTCCCCTTTCTTGCG

PTEN F

GGACGAACTGGTGTAATGATATG

PTEN R

TCTACTGTTTTTGTGAAGTACAGC

SDF-1S

GGGGGAATTCCATGAACGCCAAGGTCGTGGTC

SDF-1 AS

GGGGTCTAGAGGGCATGGATGAATATAAGCTGC

Sox2 S

ACCGGCGGCAACCAGAAGAACAG

Sox2 AS

GCGCCGCGGCCGGTATTTAT

TIMP1 F

ACT GGA AGC CCT TTT CAG AGC

TIMP1 R

AAT TCC GAC CTC GTC ATC AGG

VEGF AS

CGATCGTTCTGTATCAGTCTTTCC

VEGF S

GAAGTGGTGAAGTTCATGGATGTC