Table 2 Primer sequences used for real-time PCR
From: The differential expression pattern of the BMI-1, SALL4 and ABCA3 genes in myeloid leukemia
Primer | Sequence | Accession No | PCR product size |
|---|---|---|---|
SALL4-f | 5’-TGCAGCAGTTGGTGGAGAAC-3’ | NM_020436.3 | 68 bp |
SALL4-r | 5’-TCGGTGGCAAATGAGACATTC-3’ | ||
BMI-1-f | 5’-TAAGCATTGGGCCATAGT-3’ | NM_005180.8 | 140 bp |
BMI-1-r | 5’-ATTCTTTCCGTTGGTTGA-3’ | ||
ABCA3-f | 5’-CTCCGAGAAGGACTTTGAGG-3’ | NM_001089.2 | 144 bp |
ABCA3-r | 5’-TCCGTGTGTAACTGAACCGT-3’ | ||
β2M-f | 5’-TACACTGAATTCACCCCCAC-3’ | J00105 | 144 bp |
β2M-r | 5’-CATCCAATCCAAATGCGGCA-3’ |