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Figure 1 | Cancer Cell International

Figure 1

From: Unique functions of CHK1 and WEE1 underlie synergistic anti-tumor activity upon pharmacologic inhibition

Figure 1

Restraint of cell proliferation by WEE1 inhibitor is potentiated by a CHK inhibitor, and vice versa. A, The WEE1 inhibitor MK-1775 was titrated over A2058 cells in addition to either vehicle (DMSO), or the indicated fixed concentration of the CHK1 inhibitor, MK-8776. Tabulated EC50 concentrations for MK-1775 in the presence of vehicle or 150 nM fixed concentration of MK-8776 are presented in the lower panel for seven different cell lines. Fold change in EC50 values between vehicle treated and MK-8776 treated cells illustrates the potentiation of MK-1775 by MK-8776. B, Experiment was performed on A2058 cells as described in (A) with the exception that MK-8776 was titrated over either vehicle (DMSO) or fixed concentrations of MK-1775. C, As described in (A), A2058 cells were treated with a gradient of MK-1775 in the added presence of vehicle or 150 nM fixed concentration of MK-1775 or MK-8776.

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