Figure 2

MK-1775 combined with MK-8776 results in G1/S-phase accumulation. Cancer cell lines or primary human epithelial cells were treated with vehicle control (DMSO), 150 nM MK-1775, 500 nM MK-8776, or both compounds for 72 hours and analyzed for cell viability (top panels). Values are normalized and graphed as viable percentage of treated cells relative to DMSO treated control cells. Bottom panels, cells were treated with the same concentrations of MK-1775 and MK-8776 used in the upper panels but only for 24 hours before being harvested and analyzed by flow cytometry for DNA content.