Figure 3
Cellular p53 status does not predict synergy when MK-1775 is combined with MK-8776. A, Proliferation assays were performed on cell lines treated with four concentrations each of MK-1775 and MK-8776 titrated against each other (see Additional file 1: Figure S1). Synergy, calculated as the volumetric difference between the observed response and the predicted additive response (Bliss), was plotted for p53 wild type cells (n=15) and cell lines harboring p53 mutations (n=16). B, As described in part A, except that fractional viability relative to DMSO treated control cells from the proliferation assay was plotted for cell lines treated with 150 nM MK-1775 and 350 nM MK-8776. C, Parental and p53-deleted HCT116 and RKO matched pair cells were treated in a proliferation assay with MK-1775 in the presence of either added vehicle (DMSO) or 150 nM MK-8776. Results from the response curves were tabulated and synergy determined by fold change in MK-1775 EC50 values.