Figure 7
From: HOXB1 restored expression promotes apoptosis and differentiation in the HL60 leukemic cell line
HOXB1 promoter methylation in HL60 versus granulocytes and monocytes. Relative percentage of DNA hypermethylated (HM), not methylated (UM), and intermediate methylated (IM) fractions (a). HM and UM in 5-AzaC 1 μM treated HL60 cells after 48 h and 72 h (b). RT-PCR evaluation of HOXB1 in 5-AzaC- and TSA-treated HL60 cells compared with lymphocytes, granulocytes and monocytes (c). GAPDH is shown as quantitative standard. Western blotting of HDAC4 and HOXB1 in TSA-treated HL60 cells (d). Actin was used as quantitative standard.