Table 1 PCR primers and oligonucleotide sequences of constructs in luciferase reporter assay
Targets | Type | Primers | Oligonucleotide sequence (5′-3′) |
|---|---|---|---|
SMAD4 | |||
(site 1, site 2) | WT1 | Sense | CACAACTCGAGAGGCACAAGGTTGGTTGCTA |
Antisense | GGAAAAAAGCGGCCGCGACCTTCTGAGCAAGGCAGT | ||
SMAD4 | WT2 | Sense | CACAACTCGAGTGTGTGACACCACCCTCCTA |
(site3) | Antisense | AAGGAAAAAAGCGGCCGCTCAATCCAAGCCCGTGAGTC | |
SMAD4 | MUT1 | Sense | TGATGCACTGAATTTTTGGTATAATGTTTAAATCATGT |
(site1) | Antisense | CCAAAAATTCAGTGCATCAAATCAAGTACAAAAATA | |
SMAD4 | MUT2 | Sense | TGGCACACTGAATGTATAGAGAATTTAAGTAGAAAAGTT |
(site2) | Antisense | TATACATTCAGTGTGCCAATTGATATGATCATTGATGG | |
SMAD | MUT3 | Sense | GATTAACACTGAATGGCTGGATCATTCAGAGCTCTCTTCT |
(site3) | Antisense | GCCATTCAGTGTTAATCAAAATGGACCTAAAAAGAGCCA | |