Fungal taxol- and baccatin III- induced loss of mitochondrial membrane potential (ΔΨ
) in JR4-Jurkat cells determined by JC-1 staining and flow cytometry. Jurkat cells were incubated with either fungal taxol (6 nM) or baccatin III (3.5 μM) for indicated time periods. Mitochondrial membrane potential was analyzed using JC-1 staining. Results are given in percent of 10,000 events counted in FACS analysis. In dot plots of JR4-Jurkat cells, lower right and left quadrant (FL-1) represent the percentage of apoptotic cells that emit only green fluorescence while upper right and left quadrant show the healthy population. The percentage of cells having ΔΨm was calculated taking the ratio of red to green fluoresence against time. 2,4-DNP was used as positive control. Data are expressed as mean ± SD from three independent experiments.