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Figure 5 | Cancer Cell International

Figure 5

From: Inhibition of cancer cell proliferation and apoptosis-inducing activity of fungal taxol and its precursor baccatin III purified from endophytic Fusarium solani

Figure 5

Effect of caspase inhibitors on fungal taxol-/baccatin III- induced apoptosis. JR4-Jurkat cells (0.25 × 106) were treated with 6 nM fungal taxol (TFUNG) or 3.5 μM fungal baccatin III (BFUNG) in the presence or absence of indicated concentrations of inhibitors of (A) pancaspase (Z-VAD-FMK), (B) caspase-3 (Z-DEVD-FMK), (C) caspase-2 (Z-VDVAD-FMK), (D) caspase-9 (Z-LEHD-FMK) or (E) caspase-10 (Z-AEVD-FMK) for 48 h and assayed with PI staining followed by flow cytometry. The percentage of hypodiploid cells (sub-G1 peak) was calculated on the basis of the respective histograms. Data is expressed as mean ± SD from three independent experiments.

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