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Figure 6 | Cancer Cell International

Figure 6

From: Inhibition of cancer cell proliferation and apoptosis-inducing activity of fungal taxol and its precursor baccatin III purified from endophytic Fusarium solani

Figure 6

Apoptotic effect of fungal taxol and baccatin III as determined by Hoechst or Acridine orange-ethidium bromide dual staining. HeLa and JR4- Jurkat cells were cultured on sterile coverslips and treated with indicated concentrations of fungal taxol or baccatin III for 12 h following which the cells were stained with either Hoechst 33258 (A-C) or Acridine orange-Ethidium bromide (D-F, G-I) and viewed under a fluorescence microscope. Arrows indicate cells in early apoptosis (EA), late apoptosis (LA), live cells (LC) and convoluted nuclei. Scale bar represents 20 μm.

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