Effect of CpG-ODN and/or 5-FU treatment on HepG2 cells cycle distribution. A: Cells were incubated with CpG-ODN and 5-FU alone or together at indicated concentration for 48 h, and the cell cycle distribution was evaluated using PI staining and flow cytometry analysis. One representative flow cytometry analysis of cell cycle distribution. B: The percentage of cells in G0/G1, S and G2/M phase is expressed as mean ±SD of three independent experiments. *P<0.05,***P<0.001 versus medium. ###P<0.001 versus 5-FU. In this figure, 5-FU, CpG(2) and CpG(4) represent that the concentration of 5-FU, CpG-ODN and CpG-ODN is 7.5 μg/ml , 2 μM , 4 μM , respectively.