Effect of CpG-ODN and/or 5-FU treatment on anti-apoptotic factors within HepG2 cells. A and B: The cells seeded in six-well plates were treated with the indicated concentration of CpG-ODN (A) or 5-FU (B). After 48 h, total RNA was extracted for Bcl-2 mRNA expression using Real-time quantitative PCR. C and D: The cells seeded in six-well plates were treated with the indicated concentration of CpG-ODN and 5-FU alone or together, the Livin(C) and Survivin(D) mRNA expression were analyzed using Real-time quantitative PCR analysis as described above. Results are presented as mean ±SD of three separate experiments. *P<0.05, ***P<0.001 versus medium. #P<0.05, ##P<0.01, ###P<0.001 versus 5-FU. In this figure, 5-FU, CpG(2) and CpG(4) represent that the concentration of 5-FU, CpG-ODN and CpG-ODN is 7.5 μg/ml, 2 μM, 4 μM, respectively.