Low doses of lupeol promoted the viability and activated the PI3K/Akt pathway in HCC cell lines. (A) The chemical structure of lupeol. (B) MTT assay was performed to measure cell viability of HepG2 and SMMC7721 cells at 48 h after lupeol treatment. (C) PI3K subunit p110 protein expression and the phosphorylated level of Akt was measured by western blot in SMMC7721 cells treated with low doses of lupeol at doses of 10, 20, 30 μmol/L for 48 h. Equal loading was confirmed by stripping immunoblots and re-probing for β-actin. The quantification results were also shown and the statistical analysis was performed. Data shown were representatives of three independent experiments. * p < 0.05.