Figure 2

Synergistic anti-HCC effect of S14161 and lupeol in vitro . (A) The chemical structure of S14161. (B) MTT assay was performed to measure cell growth inhibition rate of SMMC7721 at 48 h after S14161 treatment. MTT assay was performed to measure cell growth inhibition rate of (C) SMMC7721 cells and (D) HepG2 cells 48 h after treated with lupeol alone or in combination with 1 or 3 μmol/L S14161. Expression level of PI3-kinase subunit p110 and the phosphorylated level of Akt in (E) SMMC7721 cells and (F) HepG2 cells were measured by western blot after single or combination treatment with low dose of lupeol (20 μmol/L) and S14161 (1 μmol/L or 3 μmol/L) for 48 h. Equal loading was confirmed by stripping immunoblots and re-probing for β-actin. Statistical analysis was performed. Results shown were the representatives of three independent experiments. * p < 0.05.