Synergistic anti-HCC effect of S14161 and lupeol in vivo . (A) Tumor volumes after single or combination treatment with 20 mg/kg lupeol and 20 mg/kg S14161. SMMC7721 xenograft nude mice were treated with lupeol and/or S14161 for 3 weeks, and then were observed for one more week. Tumors were measured and tumor volumes were calculated. (B) The growth inhibition rates of tumors after single or combination treatment with 20 mg/kg lupeol and 20 mg/kg S14161. (C) Body weights of mice in each group were measured every week for 5 weeks during the treatment. Values represent means ± SD of n = 6. Results shown were the representatives of three independent experiments.