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Figure 1 | Cancer Cell International

Figure 1

From: Targeted inhibition of heat shock protein 90 disrupts multiple oncogenic signaling pathways, thus inducing cell cycle arrest and programmed cell death in human urinary bladder cancer cell lines

Figure 1

Reduction of bladder cancer cell cycle activity in response to geldanamycin. (A) Representative FACS analysis of RT4 and T24 human urinary bladder cancer cells. Total cell sub-population percentages at each cell cycle phase are shown. FACS experiments were repeated three times. (B) Detection of protein (Cdk4) and mRNA expression (Cyclin D1) levels of the Cyclin/Cdk complex -critical- components responsible for the G1→S transition of cell cycle in RT4 and T24 cells. Left panel: images of protein (top) and RNA transcript (bottom) expression profiles after geldanamycin administration. Right panel: protein (top) and RNA transcript (bottom) densitometric quantification bars, denoting the drug-induced alterations of Cdk4 and Cyclin D1 expression levels compared to control conditions, using Actin (upper left panel) and GAPDH (lower left panel) as protein and gene of reference, respectively. (C) pRb and E2F1 protein expression profiles in response to 24 hours of geldanamycin treatment in RT4 and T24 bladder cancer cells. Left panel: images of protein expression profiles after geldanamycin administration. Right panel: protein densitometric quantification bars, denoting the drug-induced alterations of pRb (top) and E2F1 (bottom) expression levels compared to control conditions, using Actin (left panel) as protein of reference. Western blotting and sqRT-PCR experiments were carried out three times, one of which is respectively shown here. Standard deviation values are depicted as error bars on top of each value.

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Cancer Cell International

ISSN: 1475-2867

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