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Figure 5 | Cancer Cell International

Figure 5

From: Targeted inhibition of heat shock protein 90 disrupts multiple oncogenic signaling pathways, thus inducing cell cycle arrest and programmed cell death in human urinary bladder cancer cell lines

Figure 5

Hsp90 harm after exposure of bladder cancer cells to geldanamycin. Western blottings (upper left panel) of critical members of the eukaryotic chaperosome (Hsp90 and Hsp70) after 24-hour geldanamycin administration in RT4 and T24 bladder cancer cells. A cell line-specific Hsp90 proteolytic processing is observed, as documented by the production of a ~65 kDa Hsp90-like protein fragment. Detection of Hsp90α and Hsp90β mRNA levels via sqRT-PCR (lower left panel), proving the absence of Hsp90 transcriptional regulation in response to the drug. Right panel: protein (top and middle) and RNA transcript (bottom) densitometric quantification bars, denoting the drug-induced alterations of Hsp90/Hsp70/α-Tubulin and Hsp90α/Hsp90β expression levels compared to control conditions, using Actin (upper left panel) and GAPDH (lower left panel) as protein and gene of reference, respectively. Western blotting and sqRT-PCR experiments were executed three times, with a respective characteristic image collection presented here. Standard deviation values are depicted as error bars on top of each value.

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Cancer Cell International

ISSN: 1475-2867

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