Figure 9

Impairment of bladder cancer cell motility and invasion mechanisms. (A) Top panel: total and phosphorylated protein expression levels of the hepatocyte growth factor receptor (c-Met), upon 24-hour incubation of RT4 and T24 bladder cancer cells with increasing doses of geldanamycin, as shown by Western blotting. Bottom panel: protein densitometric quantification bars, denoting the drug-induced downregulation of total and phosphorylated c-Met expression levels, compared to control conditions, using Actin (top panel) as protein of reference. All Western blottings were performed three times, with a characteristic image collection presented here. Standard deviation values are depicted as error bars on top of each value. (B) Scratch-wound assays were carried out in RT4 and T24 bladder cancer cells under control conditions or treatment with 10 μM of geldanamycin for 24 hours. Observations were made under an inverted microscope (Carl Zeiss Axiovert 25) and pictures were taken at 20x magnification. All assays were repeated three times, while a representative image collection is shown here.