MiR-99a Target genes prediction, selection and verification. (A, B) The putative miR-99a target sequences in CTDSPL and TRIB2 genes respectively. (C) When CTDSPL or TRIB2 cloned into pGL3 vector was co-transfected into HEK-293 T cells with miR-99a precursor which had been cloned into pCD6.2 vector, luciferase activity was suppressed by 51-53% or 45-49% respectively. (D, E) MiR-99a target genes verification. Both the wild type and the full mutation of CTDSPL and TRIB2 were synthesized. When the wild-type of CTDSPL or TRIB2 was co-transfected with miR-99a precursor into HEK-293 T cells, luciferase activity was suppressed by 58-61% or 53-54% respectively. (F, G) Further verification of miR-99a Target genes (CTDSPL and TRIB2) through luciferase reporter assay in K562 cells by electroporation. Error bars represent standard deviation and each data was obtained from three independent experiments. *P < 0.05. **P < 0.01.