Figure 2

Effects of fluvastatin on activation of apoptosis-related molecules. Lymphoma cells were incubated with fluvastatin (0–10 μM) for 24 h. (A and B) Expression of cleaved caspase-3 in A20 cells (A) and EL4 cells (B) were examined by using Western blotting. Activation levels of caspase-3 were evaluated by the ratio of chemiluminescent signals between cleaved caspase-3 and β-actin. Representative images were shown in lower panel. Up panel showed the ratio of chemiluminescent signals between cleaved caspase-3 and c Data are presented as mean ± SEM of three separate experiments, *p < 0.05, **p < 0.01 versus resting cells. (C and D) Expression of Bax and Bcl2 in A20 cells (C) and EL4 cells (D) were examined by using Western blotting. Apoptosis levels were evaluated by the ratio of chemiluminescent signals between Bax and Bcl2. Representative images were shown in lower panel. Up panel showed the ratio of chemiluminescent signals between Bax and Bcl2. Data are presented as mean ± SEM of three separate experiments, **p < 0.01 versus resting cells. (E) Activity of caspase-3 in A20 cells was evaluated by using caspase-3 activity kit. Results are presented as mean ± SEM of three separate experiments, **p < 0.01 versus resting cells.