Figure 6

Fluvastatin-induced apoptosis and autophagy were blocked by mevalonate, FPP, and GGPP. (A and B) A20 lymphoma cells were incubated with fluvastatin (2.5 μM) in the presence or absence of mevalonate (Mev), farnesyl pyrophosphate (FPP), geranylgeranyl pyrophosphate (GGPP), and coenzyme Q10 (CoQ10) for 24 h. The expression of cleaved caspase-3 and LC3 were then detected by Western blotting. (A) Representative images were shown. (B) Activation of cleaved caspase-3 (Bi) and LC3 (Bii) were evaluated as described above. Data are presented as mean ± SEM of three separate experiments, **p < 0.01 versus fluvastatin group. (C) A20 lymphoma cells were incubated with fluvastatin (2.5 μM) in the presence or absence of Mev (200 μM), FPP (10 μM), and GGPP (10 μM) for 24 h. DNA fragmentation was then analyzed as described in the Methods section. Left panel showed the representative image of DNA fragmentation. The DNA fragmentation levels were evaluated by the signal intensity ratio between degraded DNA bands versus intact DNA bands (right panel). Data are presented as mean ± SEM of three separate experiments, **p < 0.01 versus fluvastatin group.