Figure 2

Real-time PCR and western blot analysis of HERC4 expression in 32 paired breast cancer and adjacent normal breast tissues. (A) Western blot indicated significant up-regulation in breast cancer tissues (T1, T2, T3, T4) in comparison with in the adjacent nontumorous breast tissues (N1, N2, N3,N4). β-actin was used as internal control. (B) Western blot was calculated as optical density value and expressed graphically. Significant differences of HERC4 protein expression between tumor (T) and adjacent nontumorous tissues (N) were analyzed statistically using the ratio between the optical densities of HERC4 and β-actin. HERC4 protein expression was significantly higher in breast cancer tissues (P < 0.05). (C) Significant differences of HERC4 mRNA level between tumor (T) and adjacent nontumorous tissues (N) were analyzed statistically by 2^-ΔΔct method. HERC4 mRNA expression was significantly higher in breast cancer tissues (P < 0.05). GAPDH was used as internal control.