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Figure 2 | Cancer Cell International

Figure 2

From: Wilms’ tumour suppressor gene 1 (WT1) is involved in the carcinogenesis of Lung cancer through interaction with PI3K/Akt pathway

Figure 2

Effect of WT1 siRNA transfection on the cell growth and cell cycle distribution of lung cancer cells, some pro-apoptotic or anti-apoptotic genes expression in A549 cancer cells. A, B. A549 cells were transfected with the WT1 targeted siRNA for 24 h, RT-PCR and western blot were performed to detect the changes of the WT1 expression level. NC: Negative control siRNA; siWT1: siRNA target WT1. The P value shows the difference between siRNA transfected cells and parental cells; bars, SE; * = P < 0.05, ** = P < 0.01. C. A549 and PC14 lung cancer cells were transfected with WT1 siRNA or negative control siRNA, 72 h post transfection, MTT was performed to detect the cell proliferation. NC: Negative control siRNA; siWT1: siRNA target WT1. D. 24 h post transfection, the cell cycle profile was monitored by FACS analysis. The distribution of cells in the G1, S, and G2/M phases of the cell cycle were calculated and labeled. NC: Negative control siRNA; siWT1: siRNA target WT1. E. A549 cells were transfected with WT1 siRNA for 24 h, and then harvested and subjected to the Western blots for the pre-apoptotic genes of BAX, BAK, p21, anti-apoptotic genes Mcl-1, Bcl-xl, Bcl-W. NC: Negative control siRNA; siWT1: siRNA target WT1. The P value shows the difference between siRNA transfected cells and parental cells; bars, SE; * = P < 0.05, ** = P < 0.01.

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