Figure 3

Reduction of WT1 mRNA and protein expression post DDP treatment in A549 cells. A. A549 cells were incubated in the present of 1 μg/ml or 2 μg/ml of DDP for different time points of 24 and 48 h, and the WT1 expression was detected by conventional RT-PCR method. The P value shows the difference between DDP treated cells and parental cells; bars, SE; * = P < 0.05, ** = P < 0.01. B. A549 cells were incubated in the present of 1 μg/ml or 2 μg/ml of DDP for different time points of 24 and 48 h, the WT1 expression was detected western blots. The P value shows the difference between DDP treated cells and parental cells; bars, SE; * = P < 0.05, ** = P < 0.01.