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Figure 4 | Cancer Cell International

Figure 4

From: Wilms’ tumour suppressor gene 1 (WT1) is involved in the carcinogenesis of Lung cancer through interaction with PI3K/Akt pathway

Figure 4

The effect of DDP treatment on PI3K/AKT pathway activity. A. A549 cells were incubated with 1 or 2 μg/ml of DDP for 24 h and 48 h, respectively, the total AKT and p-AKT was detected by western blot. The P value shows the difference between DDP treated cells and parental cells; bars, SE; * = P < 0.05, ** = P < 0.01. B. A549 cells were treated with 5 or 10 μmol of PI3K pathway inhibitor Ly294002 for 24 h, cells were then collected, cell cycle profile was monitored by FACS analysis. C. A549 cells were treated with 5 or 10 μmol Ly294002 for 24 h, cells were then collected, western blot methods were used to detect the WT1, p-AKT, and AKT expression. The P value shows the difference between Ly294002 treated cells and parental cells; bars, SE; * = P < 0.05, ** = P < 0.01. D. A549 cells were transfected with AKT-1 targeted siRNA for 24 h, cells were collected, western blot was used to detect the AKT and WT1 expression.NC: Negative control siRNA transfected cells; A549-siAKT: siRNA target AKT transfected cells. The P value shows the difference between siRNA transfected cells and parental cells; bars, SE; * = P < 0.05, ** = P < 0.01. E. a. A549 cells were transfected with WT1 targeted siRNA or control siRNA for 24 h, cells were collected, western blot was used to detect the p-AKT and WT1 expression. A549- siWT1: siRNA target WT1 transfected cells. b. 293T cells were transfected by pcDNA3.1-WT1 or control vector pcDNA3.1 for 24 h, cells were collected and then western blot assay was used to detect the expression level of p-AKT and WT1. 293T-pcDNA3.1: pcDNA3.1 transfected 293T cells; 293T-pcDNA3.1-WT1: pcDNA3.1-WT1 transfected 293T cells. The P value shows the difference between transfected cells and parental cells.

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