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Figure 6 | Cancer Cell International

Figure 6

From: Wilms’ tumour suppressor gene 1 (WT1) is involved in the carcinogenesis of Lung cancer through interaction with PI3K/Akt pathway

Figure 6

Effects of WT1 siRNA in combination with DDP treatment on cell proliferation and apoptosis. A. A549 cells were transfected with WT1 siRNA for 24 h, and then treated the cells with different concentrations of DDP for another 48 h, and cell viability was then analyzed by MTT assay. B. DDP-induced apoptosis in different A549 cells was assessed by Annexin V-PI staining. A549 cells were transfected with si-WT1 for 24 h, and then treated with or without 1 μg/ml of DDP for 24 h, cells were dual-stained with Annexin V and PI, apoptotic cells were then analyzed by flow cytometry. Three independent experiments were performed and all gave similar results. The percentage of apoptotic cells was calculated with WinMDI software.

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