Expression of CD44 in patients’ samples and leukemic cells. (A/B) Expression patterns of CD44 in leukemic patients’ samples were analyzed by PCR and real-time quantitative PCR analysis. PCR reactions were performed on templates of cDNA from different patients’ cells using a set of primers as in Materials and methods. (C) The protein expressions of CD44 in different patients were analyzed by western blotting. (D) The relative protein expression of CD44 compared to GAPDH. (E) Real-time quantitative PCR analysis of CD44 gene expression in normal volunteers and K562 cells. (F) The protein expressions of CD44 in normal controls and K562 cells. (G) The relative protein expression of CD44 compared to GAPDH. For real-time quantitative PCR and western blotting, GAPDH was used as an internal control. *, P < 0.05, **, P < 0.01, compared with the control.