Decreased proliferation in K562 cells upon CD44 silencing. (A/B) Down-regulation of CD44 mRNA/protein expression in K562 cells after CD44 interference. (C) Confocal laser microscope showing localization of CD44 in K562 cells after transfection. The green panel is GFP; the red panel is CD44 and the blue panel is cell nuclear. (D) Effect of CD44 shRNA on the proliferation of K562 cells as assessed by MTT assay. (E) CD44 surface expression detected by flow cytometry. CD44 (red), isotype control (green). Results are shown as mean ± SD of three independent experiments, each experiment in triplicate. *, P < 0.05, **, P < 0.01, compared with the control.