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Figure 3 | Cancer Cell International

Figure 3

From: Role of the Hypoxia-inducible factor-1 alpha induced autophagy in the conversion of non-stem pancreatic cancer cells into CD133+ pancreatic cancer stem-like cells

Figure 3

Enhanced HIF-11α and autophagy related proteins express level in non-stem cancer cells under intermittent hypoxia condition. (A) Isolated non-stem cancer cells were treated with intermittent hypoxia (IH) and normoxia (N) for the indicated times (24 h, 48 h and 72 h). Expression of HIF-1α, LC3-II and Beclin proteins in non-stem pancreatic cancer cells under the intermittent hypoxia (IH) and normoxia (N) conditions were detected by Western blotting. Data were normalized to β-actin levels. Experiments were repeated three times with similar data. (B) Blockade of HIF-1α with siRNA inhabited autophagy in intermittent hypoxia. Expression of HIF-1α, LC3-II and Beclin proteins in non-stem pancreatic cancer cells under the intermittent hypoxia (IH) and normoxic (N) conditions were detected after transfections by Western blotting.

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