Hydrogen peroxide (H 2 O 2 ) induces leukemic but not normal hematopoietic cell death in a dose-dependent manner

Table 1 H ₂ O ₂ leukemic primitive cell clonogenic capacity

A methylcellulose clonal assay was used to quantify clonogenic capacity of primitive cells obtained from BM, UCB and HL-60 lineage. Clonogenic capacity of BM was increased, showing that H₂O₂ induced the improvement of the capability of these cells to generate their progeny. UCB colonies decreased around 70% while HL-60 stimulated with H₂O₂ was not able to generate clones. The data are expressed as the mean ± SEM of 4 experiments. *P< 0.05, ANOVA.