| Unstimulated | 5 μM H2O2 |
---|
BM
| 68 ± 2 | 86 ± 7* |
UCB
| 45 ± 4 | 12 ± 2* |
HL-60
| 310 ± 4 | 0* |
- A methylcellulose clonal assay was used to quantify clonogenic capacity of primitive cells obtained from BM, UCB and HL-60 lineage. Clonogenic capacity of BM was increased, showing that H2O2 induced the improvement of the capability of these cells to generate their progeny. UCB colonies decreased around 70% while HL-60 stimulated with H2O2 was not able to generate clones. The data are expressed as the mean ± SEM of 4 experiments. *P< 0.05, ANOVA.