|
Unstimulated
|
5 μM H2O2
|
---|
BM
|
68 ± 2
|
86 ± 7*
|
UCB
|
45 ± 4
|
12 ± 2*
|
HL-60
|
310 ± 4
|
0*
|
- A methylcellulose clonal assay was used to quantify clonogenic capacity of primitive cells obtained from BM, UCB and HL-60 lineage. Clonogenic capacity of BM was increased, showing that H2O2 induced the improvement of the capability of these cells to generate their progeny. UCB colonies decreased around 70% while HL-60 stimulated with H2O2 was not able to generate clones. The data are expressed as the mean ± SEM of 4 experiments. *P< 0.05, ANOVA.