Figure 2

Induction of stellate morphology by TGF-β1 and Col-1. A549 and its more aggressive derivative A549LC cells were cultured in rBM 3-D culture (Mat) with or without supplementation of TGF-β1 and/or Col-1. A) Morphogenesis of A549 cells in various culture conditions were visualized by filamentous actin staining and confocal fluorescent microscopy. The images were captured at 100x. B) Similar to part A except that the images were captured from A549LC cells at 200x. C) Phase-contrast microscopy was used to capture images of A549 cells cultured in rBM 3-D (Mat) exposed to the indicated combinations of TGF-β1, Col-1, and PP2 (100x). D) The variants of A549LC cells, A549LCvec and A549LCdnSrc were cultured in rBM 3-D (Mat) in the present or absence of TGF-β1+Col-1 and morphogenesis was monitored as described in part C.