Figure 5

Klotho inhibited phosphorylation of proteins. A) Klotho expression in GC-7901 cells transfected with klotho expression vector. B) Representative of Western blot of klotho protein expression in GC-7901 cell. C) Relative klotho levels in B). D) Western blot of protein expression and phosphorylation in GC-7901 cell. p-IGF-1R: phospho-IGF-1 receptor; p-Akt: phospho-Akt; p-PI3K: phospho-PI3K; p-IRS-1: phospho-IRS-1; p-mTOR; phospho-mTOR; klotho-V: klotho expression vector; blank-V: blank vector.