The roles of apoptosis and autophagy inhibitors on autophagy. A) Immunofluorence staining of LC3-II expression. The LC3-II positive staining (green) located in the cytoplasm. The blue nuclear was stained by DAPI. GC-7901 cells were transfected with blank vector (blank-V), klotho expression vector (klotho-V), or klotho vector plus 3-MA (k-V + 3-MA), or Z-VAD-PMK (k-V + ZVP). B) Western blot of LC3-I and LC3-II expression.