Table 3 Primers and PCR amplification condition
Genes | Primers | PCR condition | |
|---|---|---|---|
|  | Sense (5′-3′) | Anti-Sense (5′-3′) |  |
IL-6 | GCTTTAAGGAGTTCCTGC | GGTAAGCCTACACTTTCCA | 95°C for 10 min. 45 cycles of 95°C for 10 s, 60°C for 10s, and 72°C for 25 s |
TNFα | GTAGCCCATGTTGTAGCA | CTCGGCAAAGTCGAGATA | |
VEGF | ACTGCTGTGGACTTGAG | CAGGTGAGAGTAAGCGA | |
MMP2 | GCAAGTTTCCATTCCGC | GTCGTCATCGTAGTTGGC | |
GAPDH | GACCCCTTCATTGACCTCAAC | CTTCTCCATGGTGGTGAAGA | |
HIF1α | GTTTACTAAAGGACAAGTCACC | TTCTGTTTGTTGAAGGGAG | 95°C for 15 min, 40 cycles of 95°C for 10s, 60°C for 45 s |
HIF2α | GTCACCAGAACTTGTGC | CAAAGATGCTGTTCATGG | |
GSK3β | CTAAGGATTCGTCAGGAACAG | TTGAGTGGTGAAGTTGAAGAG | 94°C for 3 min, 40 cycles of 94°C for 15 s, 60°C for 30s, 72°C for 30s |
RhoC | TCCTCATCGTCTTCAGCAAG | GAGGATGACATCAGTGTCCG | 30 cycles of 94°C for 30s, 58°C for 1 min,72°C for 1 min |
β 2 -microglobulin | ACCCCCACTGAAAAAGATGA | ATCTTCAAACCTCCATGATG | |
USP6 | TCAGAAGAGTGTTGCCCCAT | GGCTTTTCATGGACTCGGTT | 95°C for 3 min, 30 cycles of 94°C for 30s, 58°C for 1 min, 72°C for 1 min |
SRGAP | GGATTCCCGAAGTGACAAGC | GACTGCAGCTGGTGATAACG | |
LRRK2 | TGGGTTGGTCACTTCTGTGC | CATTGGCTGGAAATGAGTGC | |
AHNAK | GTGCCACCATCTACTTTGACA | GCTGGCTTCCTTCTGTTTGT | Â |